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flow cytometric method on dasit’s xn 1000 analytical platform  (Sysmex Corporation)

 
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    Sysmex Corporation flow cytometric method on dasit’s xn 1000 analytical platform
    Flow Cytometric Method On Dasit’s Xn 1000 Analytical Platform, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometric method on dasit’s xn 1000 analytical platform/product/Sysmex Corporation
    Average 90 stars, based on 1 article reviews
    flow cytometric method on dasit’s xn 1000 analytical platform - by Bioz Stars, 2026-03
    90/100 stars

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    Ampath Laboratories flow cytometric lymphocyte proliferation test method
    Flow <t>cytometric</t> gating strategies used to identify the response of T-cells to the N-protein and S-protein of SARS-CoV2. Gating was first set on singlet events (A), followed by gating on target populations, leucocytes (B) and then CD3 expressing T-cells (C). The stimulation of the T-cells were measured using intracellular—PE Ki-67 (D). Buffer was added to the negative control (unstimulated) in (D). The positive control in (E) was pokeweed mitogen. The stimulation of the T-cells can be seen in the <t>proliferation</t> gate (E). T-cell activation seen in the proliferation gate (F) when exposed to nucleocapsid (N) protein of SARS-CoV2. T-cell activation seen in the proliferation gate (G) when exposed to spike (S) protein of SARS-CoV2. SSC, side scatter.
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    Flow cytometric gating strategies used to identify the response of T-cells to the N-protein and S-protein of SARS-CoV2. Gating was first set on singlet events (A), followed by gating on target populations, leucocytes (B) and then CD3 expressing T-cells (C). The stimulation of the T-cells were measured using intracellular—PE Ki-67 (D). Buffer was added to the negative control (unstimulated) in (D). The positive control in (E) was pokeweed mitogen. The stimulation of the T-cells can be seen in the proliferation gate (E). T-cell activation seen in the proliferation gate (F) when exposed to nucleocapsid (N) protein of SARS-CoV2. T-cell activation seen in the proliferation gate (G) when exposed to spike (S) protein of SARS-CoV2. SSC, side scatter.

    Journal: Journal of Clinical Pathology

    Article Title: Comparison of T-cell immune responses to SARS-CoV-2 spike (S) and nucleocapsid (N) protein using an in-house flow-cytometric assay in laboratory employees with and without previously confirmed COVID-19 in South Africa: nationwide cross-sectional study

    doi: 10.1136/jclinpath-2021-207556

    Figure Lengend Snippet: Flow cytometric gating strategies used to identify the response of T-cells to the N-protein and S-protein of SARS-CoV2. Gating was first set on singlet events (A), followed by gating on target populations, leucocytes (B) and then CD3 expressing T-cells (C). The stimulation of the T-cells were measured using intracellular—PE Ki-67 (D). Buffer was added to the negative control (unstimulated) in (D). The positive control in (E) was pokeweed mitogen. The stimulation of the T-cells can be seen in the proliferation gate (E). T-cell activation seen in the proliferation gate (F) when exposed to nucleocapsid (N) protein of SARS-CoV2. T-cell activation seen in the proliferation gate (G) when exposed to spike (S) protein of SARS-CoV2. SSC, side scatter.

    Article Snippet: A standardised, in-house flow cytometric lymphocyte proliferation test method currently in use at Ampath to detect T-cell responses to other recall antigens, for example, Varicella-Zoster-Virus (VZV), Candida albicans and Tetanus toxoid was modified for the purpose of this study.

    Techniques: Expressing, Negative Control, Positive Control, Activation Assay